- Generation of Bacterial Artificial Chromosome (BAC) Transgenic Mice
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- Transgenic Mouse Methods and Protocols
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The first involves pronuclear injection into a single cell of the mouse embryo, where it will randomly integrate into the mouse genome.
The second approach, pioneered by Oliver Smithies and Mario Capecchi , involves modifying embryonic stem cells with a DNA construct containing DNA sequences homologous to the target gene. Embryonic stem cells that recombine with the genomic DNA are selected for and they are then injected into the mice blastocysts.
Generation of Bacterial Artificial Chromosome (BAC) Transgenic Mice
Genetically modified mice are used extensively in research as models of human disease. They have been used to study and model obesity, heart disease, diabetes, arthritis, substance abuse, anxiety, aging and Parkinson disease. Hundreds of these oncomice have been developed covering a wide range of cancers affecting most organs of the body and they are being refined to become more representative of human cancer.
A mouse has been genetically engineered to have increased muscle growth and strength by overexpressing the insulin-like growth factor I IGF-I in differentiated muscle fibers. Great care should be taken when deciding how to use genetically modified mice in research.
From Wikipedia, the free encyclopedia. Bibcode : PNAS American Journal of Human Genetics.
Bibcode : Sci Bibcode : Natur. Wagner; Richard D. Palmiter Genes Dev.
Transgenic Mouse Methods and Protocols
A, Plotkin, D. Therefore, you should use one of the following protocols to purify your DNA:. Using the appropriate restriction enzymes, isolate the gene fragment, including all promotor elements necessary for transcription and removing the entire plasmid backbone. This latter step is highly recommended Isolate the digested DNA on an agarose gel, making sure to run the gel long enough to cleanly separate bands which are close in size.
Purify the transgene fragment using one of the following methods:. Precipitate the DNA in ethanol.
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Belcher Sangeeta N. Cima Frank B. Jane Beil 1 ,.
Full text PDF Related articles. Abstract Transgenic mice are among the most helpful tools to study the role of genes in physiological conditions. Related articles Based on techniques.
- Transgenic Mouse Methods and Protocols | Marten H. Hofker | Springer.
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- International Society for Transgenic Technologies - Springer Books;
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